A164 IDENTIFYING THE MOST IMPORTANT PREDICTORS TO CORRELATE SERUM METABOLITES WITH MRE CHANGES IN PATIENTS WITH PEDIATRIC CROHN DISEASE

Abstract Background Endoscopy has been the gold standard for assessing activity in Pediatric Crohn disease (pCD); however, it is limited by its invasiveness and partial assessment of small intestine transmural inflammation. To that end, Inflammatory Crohn's MRE Index (PICMI) a valid, reliable, non-invasive, responsive index includes inflammation when activity. The pathogenesis pCD remains poorly understood, but evidence suggests endogenous metabolites produced intestinal tract might mediate pathogenesis. Despite important applicability metabolomics increasing understanding pCD, there research on this topic. Purpose Serum metabolomic profiles are linked to pediatric disease. Method ImageKids multicenter, prospective, observational cohort study, designed develop PICMI pCD. study was conducted over 18 months with paired serum specimens collected at initiation completion 56 patients. Due long time between visits fact during variables highly affect were not controlled, we considered each patient visit as an individual measure point. Metabolites identified using quantitative approach through Metabolomics Innovation Centre (TMIC; University Alberta). Disease determined cutoff values total score patient. most relevant medium-level high-level variable selection analysis. Pearson correlation hypothesis testing used select metabolites. Decision trees, regularization techniques, support vector machines assess explicit importance Result(s) This work provides strategy reduce dimensional dataset from experiment. By analysis able identify 117 statistical allowed indicate top 10 trough (defined index). Results, also show evaluation multivariate models dependent intrinsic model. Figure 1 reveals Tryptophan ranked highest feature scoring. Histidine, Methylhistidine, Citric acid, Isoleucine, Decanoylcarnitine correlated well severity. Image Conclusion(s) uses unique analyses, associated previously significantly altered blood IBD patients compared controls. Histidine known be involved mediation oxidative stress, potentially influencing These could serve biomarkers help define Disclosure Interest None Declared